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. Author manuscript; available in PMC: 2009 Oct 17.
Published in final edited form as: Cell. 2008 Oct 17;135(2):250–260. doi: 10.1016/j.cell.2008.09.054

Figure 3. Mre11, Rad50, HerA, and NurA resects the 5’ strand and not the 3’ strand.

Figure 3

(A) Nuclease assays were performed as in Fig. 2 except that the linearized 6.5 kb plasmid substrate was attached to a magnetic bead via a biotin/streptavidin linkage to the 5’ strand of the DNA distal to the probe region (see schematic at top). Assays were performed with 5.4 nM wild-type HerA, 38.4 nM wild-type NurA, and 6.6 nM wild-type MR as indicated for 30 min and analyzed in native agarose gels.

(B) Nuclease assays were performed as in (A) but the reaction products were analyzed using a probe specific for the 3’ strand adjacent to the 5’ biotin/bead (see schematic at top).