TABLE 1.
Suitability of oma-1(zu405) silencing assay in heritable silencing interference studies
| Test | dsRNA trigger | Recipient genotype | Animals tested | Biological effect |
|---|---|---|---|---|
| Effect of specific dsRNA | oma-1 (A1) | oma-1(zu405) | 5 | >50 progeny (5/5) |
| Effect of specific dsRNA | oma-1 (A2) | oma-1(zu405) | 5 | >50 progeny (5/5) |
| Effect of nonspecific dsRNA | gfp | oma-1(zu405) | 5 | Dead eggs only (5/5) |
| Effect of nonspecific dsRNA | unc-22 | oma-1(zu405) | 3 | Dead eggs only (3/3) |
| Effect of no dsRNA trigger | None | oma-1(zu405) | 3 | Dead eggs only (3/3) |
| Dependence on RNAi machinery | oma-1 (A1) | oma-1(zu405); rde-1(ne300) | 5 | Dead eggs only (5/5) |
We tested the response of animals carrying mutations in the oma-1 gene to determine the feasibility of the assay to study heritable silencing. Viable progeny at the restrictive temperature (23°) for animals oma-1(zu405) are the positive readout for silencing. Dead eggs only are negative readout for silencing.