TABLE 2.
Genetic and physical position of AFLP marker-anchored potato BAC clones
| BAC clone | Marker ID | Chromosome arm | cM | FLa | Relative physical locationb | n |
|---|---|---|---|---|---|---|
| RH177C17 | 20,054 | Short arm | 0.0 | 0.7 ± 0.2 | 1.2 ± 0.4 | 10 |
| RH160K03 | 7,679 | 0.8 | d | d | ||
| RH083L10 | 20,669 | 0.0–1.6 | 1.8 ± 0.3 | 3.1 ± 0.5 | 11 | |
| RH081K17 | 20,298 | 0.8–1.6 | d | d | ||
| RH097M07 | 7,688 | 1.6 | d | d | ||
| RH136O23 | 7,689 | 1.6 | d | d | ||
| 39P07 | GP79 | c | 2.4 ± 0.4 | 4.2 ± 0.7 | 7 | |
| RH084L03 | 12,631 | 3.1 | 3.2 ± 0.5 | 5.6 ± 0.9 | 7 | |
| RH034P18 | 7,717 | 7.0 | 4.7 ± 0.5 | 8.1 ± 0.9 | 14 | |
| RH125D13 | 7,717 | 7.0 | d | d | ||
| Hetero-euchromatin boundary | 5.6 ± 0.8 | 9.8 ± 1.4 | 16 | |||
| RH069B12 | 7,751 | 10.7 | 6.7 ± 0.6 | 11.6 ± 1.0 | 8 | |
| RH077N17 | 7,752 | 11.5 | 8.1 ± 1.0 | 14.0 ± 2.0 | 5 | |
| RH018J03 | 12,623 | 7.0–12.2 | 8.7 ± 1.0 | 15.1 ± 1.7 | 6 | |
| RH143A24 | 12,659 | 11.5–12.2 | d | d | ||
| RH075F10 | 7,786 | 12.2 | 10.8 ± 1.6 | 18.8 ± 2.9 | 10 | |
| RH022P07 | 7,786 | 12.2 | d | d | ||
| Centromere | 11.8 ± 0.9 | 20.5 ± 1.5 | 16 | |||
| RH082H07 | 12,647 | Long arm | 11.5–12.2 | 14.2 ± 1.8 | 24.7 ± 3.1 | 3 |
| RH130P10 | 7,801 | 12.2 | 15.2 ± 1.0 | 26.5 ± 1.7 | 10 | |
| RH060M13 | 20,173 | 12.2–14.4 | 18.0 ± 1.3 | 31.3 ± 2.2 | 5 | |
| Hetero-euchromatin boundary | 21.6 ± 1.8 | 37.5 ± 3.2 | 16 | |||
| RH139E21 | 7,753 | 11.5–12.2 | 22.1 ± 2.3 | 38.4 ± 4.0 | 12 | |
| RH057H05 | 7,891 | 13.7 | 23.3 ± 1.3 | 40.5 ± 2.2 | 5 | |
| RH160C14 | 7,905 | 15.2 | 25.2 ± 1.1 | 43.7 ± 1.9 | 12 | |
| RH188N15 | 20,129 | 15.9–16.7 | 26.3 ± 1.1 | 45.7 ± 1.9 | 12 | |
| RH094G20 | 7,933 | 20.3 | 30.2 ± 0.9 | 52.5 ± 1.6 | 4 | |
| Knob | 30.9 ± 2.4 | 53.6 ± 4.3 | 16 | |||
| RH102I10 | 12,834 | 21.4–23.3 | 33.2 ± 0.8 | 57.7 ± 1.4 | 11 | |
| RH051B02 | 20,052 | 24.9 | 33.8 ± 1.0 | 58.6 ± 1.7 | 4 | |
| RH194M18 | 7,989 | 34.3–35.0 | 38.7 ± 0.4 | 67.2 ± 0.8 | 7 | |
| RH103A21 | 8,016 | 36.5 | 40.8 ± 1.3 | 70.9 ± 2.2 | 8 | |
| RH083C08 | 8,040 | 37.3 | 41.4 ± 0.6 | 71.8 ± 1.0 | 7 | |
| RH087B02 | 8,060 | 41.2 | 44.5 ± 0.9 | 77.3 ± 1.6 | 8 | |
| RH060H14 | 8,076 | 42.0 | 46.3 ± 0.6 | 80.5 ± 1.0 | 7 | |
| RH200K19 | 12,982 | 53.7 | 49.8 ± 0.6 | 86.4 ± 1.0 | 6 | |
| RH204G08 | 12,998 | 56.8–57.6 | 52.7 ± 0.6 | 91.5 ± 1.0 | 6 |
Fraction length (FL) = (S/T) × 57.6, where S is the distance (micrometers) from the FISH site to the end of the short arm, T is the total length of the chromosome (micrometers), and 57.6 is the length (in centimorgans) of the linkage map of chromosome 6.
Relative physical location = (S/T) × 100, where S is the distance (in micrometers) from the FISH site to the end of the short arm of the chromosome, and T is the total length of the chromosome (in micrometers). All measurements were made on pachytene chromosomes 6 of average length of 41.1 ± 5.2 μm.
BAC 39P07 is anchored by marker GP79 that was mapped to 4.4 cM on the potato linkage group 6 by Tanksley et al. (1992).
The location of BAC RH177C17 on the pachytene chromosome was completely overlapped with RH160K03; RH083L10 overlapped with RH081K17, RH097M07, and RH136O23; RH034P18 overlapped with RH125D13; RH143A24 overlapped with RH018J03; and RH022P07 overlapped with RH075F10.