Figure 2.—

FISH pairing assay in germaria and embryos of control w¹¹¹⁸ flies. (A) Cartoon diagram of the germarium. Pairing was assayed collectively in C(3)G staining nuclei of regions 2a and 2b. (B) Paired dodeca foci (green), partially separated between a strand of SC, indicated by immunofluorescence (red) to C(3)G in region 2a. (C) A single focus (green) using a region 25 BAC probe indicating pairing in a meiotic nucleus of 2a. (D) A single focus (green) using a histone cluster probe indicating pairing in a meiotic nucleus of 2a. (E and F) FISH in early embryos. FISH experiments were performed on 2- to 4-hr-old embryos. Pairing was assayed in embryos that had completed cellularization and just begun gastrulation (∼3 hr). (E) Dodeca probe. (F) Histone cluster probe. In all panels blue indicates DAPI. Each panel is a single z-section.