TABLE 3.

Fold changes in mRNA abundance in UPF1 and upf1Δ strains

				Effect on abundance
NMD	ASH1 allele	Fold change ash1/ASH1	Pa	ash1 allele	NMD
UPF1	ASH1b	1.00 ± 0.08	1	None
upf1Δ	ASH1b	0.92 ± 0.25	1
upf1Δ/UPF1		0.92 ± 0.25	0.68		None
UPF1	ash1-A-ns1b	0.98 ± 0.08	0.87	None
upf1Δ	ash1-A-ns1b	4.07 ± 0.16	0.00009
upf1Δ/UPF1		4.15 ± 0.16	0.0001		Increase
UPF1	ash1-A-ns2c	0.97 ± 0.42	0.91	None
upf1Δ	ash1-A-ns2c	2.84 ± 0.55	0.0003
upf1Δ/UPF1		2.93 ± 0.57	0.002		Increase
UPF1	ash1-A-ms1c	0.72 ± 0.09	0.12	None
upf1Δ	ash1-A-ms1c	0.82 ± 0.20	0.25
upf1Δ/UPF1		1.14 ± 0.28	0.42		None
UPF1	ash1-B-ns1b	1.54 ± 0.23	0.02	Increase
upf1Δ	ash1-B-ns1b	1.52 ± 0.10	0.01
upf1Δ/UPF1		0.99 ± 0.06	0.92		None
UPF1	ash1-B-ms1c	1.21 ± 0.46	0.48	None
upf1Δ	ash1-B-ms1c	0.89 ± 0.12	0.06
upf1Δ/UPF1		0.74 ± 0.26	0.23		None
UPF1	ash1-C-ns1b	1.87 ± 0.26	0.0008	Increase
upf1Δ	ash1-C-ns1b	1.73 ± 0.28	0.005
upf1Δ/UPF1		0.93 ± 0.15	0.70		None
UPF1	ash1-C-ms1c	1.44 ± 0.67	0.27	None
upf1Δ	ash1-C-ms1c	0.85 ± 0.28	0.25
upf1Δ/UPF1		0.59 ± 0.19	0.15		None
UPF1	ash1-C-ms2c	1.18 ± 0.44	0.53	None
upf1Δ	ash1-C-ms2c	0.93 ± 0.52	0.33
upf1Δ/UPF1		0.79 ± 0.44	0.49		None

^aStatistical analyses were performed using two-tailed t-tests (materials and methods). H₀: mutant ash1 mRNA abundance equals wild-type ASH1 mRNA abundance. H₁: mutant ash1 mRNA abundance does not equal wild-type ASH1 mRNA abundance. P-values <0.05 (italic) indicate a change in abundance.

^bExperiments were performed by expressing ash1 alleles that were integrated at the ASH1 locus by gene replacement.

^cExperiments were performed by expressing ash1 alleles from a centromeric plasmid.