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The two-hybrid interaction of yKu70 and Sir4 is dependent on Sir2, but not on Sir1, Sir3, yKu80, or the endogenous copies of Sir4 or yKu70. Strains shown are DRY5492 (sir3Δ), DRY5483 (sir2Δ), DRY5474 (sir1Δ), DRY5465 (yku80Δ), DRY5447 (wild-type pGBD-HDF1 and pGAD-Sir4, aa 1205–1348), DRY5449 (pGAD-empty), DRY5448 (pGBD-empty), DRY5492 (sir4Δ), and DRY5456 (yku70Δ).
