TABLE 1.
Strains used in this study
| Strain | Genotype |
|---|---|
| DRY705 | MATα his3-11 leu2-3,112 lys2Δ∷hisG trp1-1 ura3-1 |
| DRY5430 | MATaADE2 lys2Δ∷hisG |
| DRY5431 | MATayku70Δ∷natMX4 ADE2 lys2Δ∷hisG |
| DRY5432 | MATasir1Δ∷kanMX4 ADE2 lys2Δ∷hisG |
| DRY5433 | MATayku70Δ∷natMX4 sir1Δ∷kanMX4 ADE2 lys2Δ∷hisG |
| DRY5438 | MATα yku70Δ∷natMX4 ADE2 lys2Δ∷hisG |
| DRY5439 | MATα sir1Δ∷kanMX4 ADE2 lys2Δ∷hisG |
| DRY5440 | MATα yku70Δ∷natMX4 sir1Δ∷kanMX4 ADE2 lys2Δ∷hisG |
| DRY829 | MATα HMR∷ADE2 ade2∷HIS3 his3-11 leu2-3,112 trp1-1, ura3-1 |
| DRY5406 | sir1Δ∷kanMX4 in DRY829 |
| DRY5408 | yku70Δ∷natMX4 in DRY829 |
| DRY826 | MATα HMR∷ADE2 ΔI ade2∷HIS3 his3-11 leu2-3,112 trp1-1, ura3-1 |
| DRY5414 | sir1Δ∷kanMX4 in DRY826 |
| DRY5416 | yku70Δ∷natMX4 in DRY826 |
| DRY815 | MATα HMR-SS∷ADE2 ade2∷HIS3 his3-11 leu2-3,112 trp1-1, ura3-1 |
| DRY5418 | sir1Δ∷kanMX4 in DRY815 |
| DRY5420 | yku70Δ∷natMX4 in DRY815 |
| DRY640 (JRY2334)a | MATα ade2-1 his3-11 leu2-3,112 trp1-1 ura3-1 can1-100 |
| DRY707 | MATaADE2 his3-11 leu2-3,112 lys2∷hisG trp1-1 ura3-1 |
| DRY1667 | MATα HMR∷URA3pr-ADE2 ade2∷HIS3 |
| DRY1665 | MATα HMR∷URA3pr-ADE2 ΔI ade2∷HIS3 |
| DRY5404 | MATα HMR∷URA3pr-ADE2 yku70Δ∷natMX4 ade2∷HIS3 |
| DRY5402 | MATα HMR∷URA3pr-ADE2 sir1Δ∷kanMX4 ade2∷HIS3 |
| DRY3348 | MATaTELVIIL∷URA3 ADE2 LYS2 ura3Δ0∷kanMX4 |
| DRY3762 | MATaYKU70-MYC9-TRP1 in DRY3348 |
| DRY5539 | MATα sir4Δ∷natMX4 YKU70-MYC9-TRP1 ADE |
| DRY2805 (PJ69-4A)b | MATatrp1-901 leu2-3,112 ura3-52 his3-200 gal4Δ gal80Δ LYS2∷GAL1-HIS3 GAL2-ADE2 met2∷GAL7-lacZ |
| DRY5119 | sir1Δ∷kanMX4 in DRY2805 |
| DRY5122 | sir3Δ∷kanMX4 in DRY2805 |
| DRY5125 | sir4Δ∷kanMX4 in DRY2805 |
| DRY5129 | yku70Δ∷kanMX4 in DRY2805 |
| DRY5136 | sir2Δ∷kanMX4 in DRY2805 |
| DRY5137 | yku80Δ∷kanMX4 in DRY2805 |
| DRY5447 | pDR1344 (pGBD-YKU70) and pDR1473 (pGAD-Sir4, aa 1205–1348) in DRY2805 |
| DRY5448 | pDR1473 (pGAD-Sir4, aa 1205–1348) and pDR1406 (pGBD-TRP1-empty) in DRY2805 |
| DRY5449 | pDR1344 (pGBD-YKU70) and pDR1405 (pGAD-LEU2-empty) in DRY2805 |
| DRY5456 | pDR1344 (pGBD-YKU70) and pDR1473 (pGAD-Sir4, aa 1205–1348) in DRY5129 |
| DRY5465 | pDR1344 (pGBD-YKU70) and pDR1473 (pGAD-Sir4, aa 1205–1348) in DRY5137 |
| DRY5474 | pDR1344 (pGBD-YKU70) and pDR1473 (pGAD-Sir4, aa 1205–1348) in DRY5119 |
| DRY5483 | pDR1344 (pGBD-YKU70) and pDR1473 (pGAD-Sir4, aa 1205–1348) in DRY5136 |
| DRY5492 | pDR1344 (pGBD-YKU70) and pDR1473 (pGAD-Sir4, aa 1205–1348) in DRY5122 |
| DRY5501 | pDR1344 (pGBD-YKU70) and pDR1473 (pGAD-Sir4, aa 1205–1348) in DRY5125 |
All strains are isogenic to W303-1a.
a
From J. Rine.
b
From E. Craig.