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. 2008 Nov;180(3):1429–1443. doi: 10.1534/genetics.108.091272

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Copyright © 2008 by the Genetics Society of America

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Figure 4.— — sax-7 and unc-44 genetically interact in neuronal positioning maintenance. (A) Quantitation of animals exhibiting displaced GABA neurons in sax-7, unc-44, and unc-119 mutant backgrounds. Error bar shows the standard error of the proportions of three sample sets where in each set n = 100. (B) UNC-44 and SAX-7 localizations are not interdependent. (Left) UNC-44 immunostaining. (Right) SAX-7 immunostaining in wild-type, unc-44(e362), and sax-7 (eq1) adult animals as well as arrested embryos homozygous for the nDf41 chromosomal deficiency, which removes the unc-44 gene; as expected, these embryos do not show UNC-44 staining. Images of UNC-44 and SAX-7 immunostaining in respective animals of different genetic backgrounds were taken with the same exposure times. Insets show corresponding UNC-44 and SAX-7 localization to the membrane boundaries of cells. Bar, 20 μm.