TABLE 1.
The SAX-7 cytoplasmic tail and STN-2 interact by yeast two-hybrid assays
| Growth media | SAX-7 | SAX-7 + pGADT7-53 | SAX-7 + STN-2 | SAX-7ΔPB + STN-2 | SAX-7 + STN-2ΔPDZ | STN-2 + pGBKT7-T | STN-2 |
|---|---|---|---|---|---|---|---|
| L− | ND | ND | ND | ND | ND | ND | + |
| T− | + | ND | ND | ND | ND | ND | − |
| L−/T− | − | + | + | + | + | + | − |
| L−/T−/H− | − | − | + | − | − | − | − |
| L−/T−/H−/A− | − | − | + | − | − | − | − |
The Y2H interaction between the SAX-7 cytoplasmic tail (SAX-7) and STN-2 requires the SAX-7 PB motif and the STN-2 PDZ domain. ND, not determined. +, growth; −, no growth on minimal media lacking the following specified amino acids: L, leucine; T, tryptophan; H, histidine; and A, adenine. Growth on L−/T−/H− and L−/T−/H−/A− media indicates robust positive interaction. pGADT7-53 and pGBKT7 are negative control vectors that do not interact with SAX-7 and STN-2, respectively.