Figure 2.—

Induction of plasmid-borned mraY alleles can protect against E-mediated lysis. Cultures of MDS12 tonA∷Tn10 (λ*E), bearing derivatives of the plasmid pBAD30 carrying the indicated alleles of mraY of E. coli (A) or B. subtilis (B) were induced at either A₅₅₀ = 0.2 (open symbols) or A₅₅₀ = 0.5 (solid symbols) and monitored for culture turbidity, as described in materials and methods. ○ and •, pBAD30 vector; □ and ▪, pBAD30 carrying the wt mraY gene; and ▵ and ▴, pBAD30 carrying the inactivated gene (^EcmraY_D267N or ^BsmraY_D231N).