TABLE 1.

Origin, coordinates, and neutral-genetic variation parameters for the studied populations

Habitat	Population	Coordinates		N alleles	Allelic richness	Hexp	Hobs	Fis	s
River	Buz	43.46 N	3.59 E	4.0 ± 1.6	3.53 ± 1.32	0.56 ± 0.18	0.51 ± 0.24	0.086 ± 0.097*	0.004, NS
River	Lam	43.47 N	3.43 E	4.1 ± 2.0	3.59 ± 1.46	0.59 ± 0.14	0.53 ± 0.12	0.075 ± 0.054, NS	0.005, NS
River	Lez	43.43 N	3.49 E	6.2 ± 2.7	3.99 ± 1.40	0.60 ± 0.22	0.50 ± 0.23	0.139 ± 0.062***	0.009, NS
River	Mos	43.40 N	3.46 E	6.2 ± 2.9	4.42 ± 1.84	0.64 ± 0.17	0.55 ± 0.23	0.111 ± 0.084**	0.03, NS
River	Sal	43.41 N	3.55 E	5.6 ± 2.8	4.43 ± 2.02	0.58 ± 0.26	0.53 ± 0.21	0.087 ± 0.061*	0.08, NS
Pond	Vio 01	43.44 N	3.42 E	5.2 ± 2.3	4.07 ± 1.82	0.60 ± 0.21	0.44 ± 0.28	0.203 ± 0.090***	0.30**
Pond	Vio 02	43.45 N	3.43 E	2.9 ± 0.8	2.28 ± 1.13	0.54 ± 0.17	0.39 ± 0.22	0.246 ± 0.100**	0.26, NS
Pond	Vio 07	43.45 N	3.46 E	5.3 ± 2.3	4.00 ± 1.49	0.55 ± 0.21	0.49 ± 0.20	0.087 ± 0.076*	0.008, NS
Pond	Vio 11	43.46 N	3.47 E	3.4 ± 1.2	2.94 ± 0.89	0.48 ± 0.21	0.54 ± 0.25	−0.119 ± 0.058, NS	0.03, NS
Pond	Vio 12	43.46 N	3.48 E	3.6 ± 2.1	3.01 ± 1.84	0.42 ± 0.23	0.37 ± 0.21	0.130 ± 0.072*	0.20, NS
All rivers				5.2 ± 1.1	3.99 ± 0.43	0.59 ± 0.03	0.52 ± 0.02	0.11 ± 0.03	0.002
All ponds				4.1 ± 1.1	3.26 ± 0.76	0.52 ± 0.07	0.45 ± 0.07	0.14 ± 0.17	0.17

N alleles, number of alleles; H_exp, gene diversity; H_obs, observed heterozygosity; s, selfing rate. *P < 0.05; **P < 0.01; ***P < 0.001; NS, P > 0.05.