TABLE 3.
Secondary screen for susceptibility of fly lines to bacterial infections
|
S. typhimurium (gram −)
|
L. monocytogenes (gram +)
|
|||||
|---|---|---|---|---|---|---|
| Line | Wild type | Sensitive | Resistant | Wild type | Sensitive | Resistant |
| EE2 | + | + | ||||
| GC15 | + | + | ||||
| GE5 | + | + | ||||
| iG1 | + | + | ||||
| EP2425 | + | + | ||||
| 11603 | + | + | ||||
| 12718 | + | + | ||||
| 12738 | + | + | ||||
| 12516 | + | + | ||||
| 13425 | + | + | ||||
| 13462 | + | + | ||||
| 13593 | + | + | ||||
| 13596 | + | + | ||||
| 13739 | + | + | ||||
| 13760 | + | + | ||||
| 13918 | + | + | ||||
| 13995 | + | + | ||||
| 16497 | + | + | ||||
All flies were ultimately killed by the infecting pathogen. Wild type, resistant, or sensitive refer to changes in survival relative to wild-type flies. Flies were injected with 50 nl S. typhimurium (OD600 = 0.1) or L. monocytogenes (OD600 = 0.01) and incubated at 29°. Significance was determined by log-rank test. n = 30 flies. See Figure 1 for survival curves of lines that were significantly sensitive or resistant to bacterial challenge.