Table 1.
Hematologic parameters of hem6 and control animals
| Parameter | 4 weeks |
24 weeks |
||||
|---|---|---|---|---|---|---|
| +/+ (n = 9) | hem6/hem6 (n = 8) | P | +/+ (n = 10) | hem6/hem6 (n = 8) | P | |
| WBCs, ×103/μL | 3.08 ± 1.22 | 3.94 ± 1.99 | .345 | 4.63 ± 1.14 | 6.06 ± 2.34 | .459 |
| RBCs, ×106/μL | 7.52 ± 0.62 | 9.59 ± 0.9 | < .001 | 9.59 ± 0.89 | 12.55 ± 2.13 | < .001 |
| Hemoglobin, g/dL | 128 ± 12 | 107 ± 10 | .002 | 136 ± 13 | 118 ± 21 | < .001 |
| Hematocrit, % | 46.5 ± 3.9 | 45.9 ± 5.2 | .803 | 50.7 ± 3.2 | 47.9 ± 6.3 | .075 |
| MCV, fL | 61.9 ± 1.9 | 47.7 ± 2.1 | < .001 | 53.3 ± 4.5 | 38.5 ± 1.9 | < .001 |
| MCH, pg | 17.0 ± 1.2 | 11.1 ± 0.9 | < .001 | 14.2 ± 9.4 | 9.4 ± 0.3 | < .001 |
| MCHC, g/dL | 275 ± 16 | 232 ± 24 | .003 | 266 ± 11 | 245 ± 15 | < .001 |
| RDW, % | 13.2 ± 0.9 | 18.2 ± 1.9 | < .001 | 13.8 ± 1.4 | 17.0 ± 0.5 | < .001 |
| Platelets, × 106/μL | 97.1 ± 11.7 | 91.0 ± 10.3 | .297 | 110.5 ± 12.4 | 100.2 ± 12.1 | .207 |
| MPV, fL | 7.5 ± 0.5 | 7.3 ± 0.7 | .651 | 7.4 ± 0.2 | 7.1 ± 10.4 | .236 |
| Reticulocytes, % | 7.7 ± 2.3 | 6.0 ± 1.7 | .13 | 2.1 ± 1.0 | 1.6 ± 0.4 | .408 |
| Abs retic, x106/μL | 5.7 ± 1.7 | 10.5 ± 12.0 | .342 | 2.01 ± 0.78 | 2.06 ± 0.66 | .655 |
| CHr, pg | 16.5 ± 0.3 | 12.7 ± 0.6 | < .001 | 15.2 ± 1.1 | 11.6 ± 0.5 | < .001 |
| ZnPP/H, μmol/mol | 105 ± 44 | 51 ± 12 | .010 | 64 ± 8 | 43 ± 6 | < .001 |
CBCs and RBC zinc ratios of protoporphyrin to heme (ZnPP/H) were determined in 129B6N2F1-+/+ and -hem6/hem6 animals at 4 and 24 weeks of age. Values are presented as the mean plus or minus 1 SD. P values are reported for the 2-tailed Student t test. Italics indicate significant differences.
WBC indicates white blood cell; RDW, red cell distribution width; MPV, mean platelet volume; Abs retic, absolute reticulocytes.