Table 1.
RT-PCR hybridization probes
| Gene | Ensembl transcript ID | Exon(s) | Size, bp | Forward primer | Reverse primer |
|---|---|---|---|---|---|
| α-adducin | ENSMUST00000114340 | 9 | 158 | CAAGTGCTGGAGGACCAGACAACTT | CTTATGCGGATGCTCGATAATCTGG |
| α-adducin | ENSMUST00000114340 | 10-12 | 444 | TACCCTTATCGATACCCTGCTCTGA | ATGATGGACAGAAGCCTTGTTCAGG |
| α-adducin | ENSMUST00000114340 | 15 | 498 | TGACGGCCTCCAAAGCCATCATTGA | AGGTCCTGTGTGTCCTCTCCTCATT |
| β-adducin | ENSMUST00000032069 | 12-14 | 360 | GAAGGCTGATGAAGTGGAAAAGTCC | AGAAGCTAGAACAGGAGCAGGAAGG |
| γ-adducin | ENSMUST00000050096 | 15 (3′UTR) | 704 | TAGCCAATCAGTCAGGGGTA | CTCAAAGTTTCCACTACGAG |
| actin | ENSMUST00000031564 | 3-6 | 929 | GAGGCCCAGAGCAAGAG | CCGGACTCATCGTACTC |
| neomycin | 278 | TGGAGAGGCTATTCGGCTATGACT | AGCAAGGTGAGATGACAGGAGATC |