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. 2008 Sep 10;112(10):4109–4116. doi: 10.1182/blood-2008-02-139527

Figure 2.

Figure 2

Lack of PKC-θ does not affect NK cell–mediated cytotoxicity. Ex vivo (A) and IL-2–cultured NK cells from PKC-θ−/− and WT (B-D) mice were evaluated for cytotoxicity against YAC-1 cells (A,B), RMA-S parental cells, and RMA-S cells transfected with the NKG2D ligand Rae-1γ (B), to assess NKG2D/DAP10/DAP12-independent and -dependent pathways, respectively. Cytotoxicity was measured by standard chromium release assay. IL-2–cultured WT and PKC-θ−/− NK cells were tested against parental Baf/3 cells and Baf/3 cells transfected with Hm1-C4 and m157 to assess Ly49D and Ly49H-transduced signals, respectively (C), and against anti-Thy1.2–coated EL-4 cells to assess CD16-mediated killing (antibody-dependent cellular cytotoxicity) (D).