Fig. 5.

ADP dose response for the full platelet model. (A) Relative fluorescence of Fluo-4–loaded human platelets was measured after treatment with 100 nM, 1 μM, or 10 μM ADP. (B-F) After 1 minute of simulated rest, the platelet model was activated by setting the extracellular [ADP] to 100 nM, 1 μM, or 10 μM. Deterministic computation of (B) [Ca²⁺]_i, (C) [IP₃], (D) [PLC-β*], (E) deactivated [PLC-β], and (F) activated [G_q·GTP]. (G,H) Gel-filtered human platelets were activated with ADP and maximal changes in (G) [Ca²⁺]_i and (H) [IP₃] from basal levels were measured.³⁶ For panels G and H, simulated responses are plotted in red.