Table 1.
Markers used to define HPC and lineage cells
| Hematopoietic lineage cells | Marker |
|---|---|
| T-cell lineage | |
| THY | |
| DN1 | CD25− CD44+ Lin− |
| DN2 | CD25+ CD44+ Lin− |
| DN3 | CD25+ CD44− Lin− |
| DN4 | CD25− CD44− Lin− |
| DP | CD4+ CD8+ |
| THY/SPL | |
| CD4 SP | CD4+ TCRhi |
| CD8 SP | CD8+ TCRhi |
| nTreg | Foxp3+ CD4+ CD25+ |
| NKT | TCR+ CD1d+ |
| B-cell lineage | |
| BM | |
| HPC | Sca1+ ckit+ Lin−* |
| Fr A | CD43+ B220lo CD24− |
| Fr B+C | CD43+ B220lo CD24+ |
| Fr D | CD43− B220+ IgM− IgD− CD93+ |
| Fr E | CD43− B220+ IgM+ IgD− CD93+ |
| Fr F | CD43− B220+ IgM+ IgD+ CD93− |
| SPL | |
| T1 | CD23− IgM+ CD24+ CD21−/lo |
| T2-FO | CD23+ CD24+ CD21lo |
| FO | CD23+ IgMint CD24lo CD21+ |
| T2-MZP | CD23+ IgMhi CD24+ CD21hi |
| MZ | CD23− IgM+ CD24+ CD21hi |
| PT | |
| B1a | CD19hi CD5+ B220lo |
| B2 | CD19lo CD5− B220hi |
| PPs | |
| PNA− | B220+ CD19+ PNA+ |
| PNA+ | B220+ CD19+ PNA− |
| Myeloid cells | |
| BM/SPL | |
| Imm N | Gr1lo CD11b+ |
| Mat N | Gr1hi CD11b+ |
| SPL/culture | |
| NK | Dx5+ TCR− |
| DC | CD11b+ CD11c+ |
| Mo/Ma | CD11b+ CD11c− |
| PB | |
| Platelets | CD41+ CD61+ |
B indicates B cell; B1/B2, peritoneal B cells; BM, bone marrow; culture, bone marrow cells cultured for 7 days with M-CSF or GM-CSF; DC, dendritic cell; DN, CD4CD8 double-negative; DP, CD4CD8 double-positive; FO, follicular; Fr, fraction; HPC, hematopoietic progenitor cells; Mo/Ma, monocyte/macrophage; MZ, marginal zone; N, neutrophil; NK, natural killer cell; NKT, NK T cell; nTregs, naturally occurring T cells; PB, peripheral blood; PNA, peanut agglutinin; PPs, Peyer's patches; PT, peritoneum; SP, CD4 or CD8 single-positive; SPL, spleen; T1, transitional 1 B cell; T2 transitional 2 B cell; T2-FO, transitional 2 follicular precursor B cell; T2-MZP, transitional 2 marginal zone precursor B cell; and THY, thymus.
Lin−: CD11b, CD11c, Gr-1, CD4, CD8, B220.