Figure 2.

Whole-mount in situ hybridization analysis of Kielin expression in embryos at stage 10.5 (A; vegetal view), stage 12 (B; dorsal view), stage 14 (C; dorsal view), stage 19 (D; cleared dorsal view), stage 24 (E, cleared lateral view), and stage 28 (F, cleared lateral view). Histological analyses show Kielin expression in the axial mesoderm of a late gastrula (G), in the notochord and presumptive floor plate of a midneurula (H), and in the floor-plate region of an early tailbud embryo (I). Arrowheads, stomodeal-hypophyseal anlage; am, axial mesoderm; ect, ectoderm; ae, archenteron; fp, presumptive floor plate; s, somite; nt, notochord.