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Regulation of Kielin expression in the marginal zone explant. VMZ explants were prepared from embryos injected with control lacZ mRNA (300 pg; A), Chd mRNA (100 pg; B), Xnr-4 mRNA (200 pg; C), goosecoid mRNA (50 pg; D), HNF-3β mRNA (100 pg; E), and Xnot mRNA (300 pg; F). The explants were prepared at stage 10.5, harvested at stage 12.5, and analyzed by in situ hybridization with a Kielin probe.
