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Overexpression of Kielin expands the MyoD expression region in vivo. Two left vegetal blastomeres of eight-cell embryos were injected with frame-shift Kielin (control) mRNA (600 pg; A, C, and E) and wild-type Kielin (KLN) mRNA (600 pg; B, D, and F). The injected embryos were harvested at stage 16 (A and B), stage 13 (C and D), or stage 19 (E and F) and analyzed by whole-mount in situ hybridization with a MyoD probe (A and B), a Xnot probe (C and D), or a NCAM probe (E and F).
