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. 2000 Apr 25;97(10):5291–5296. doi: 10.1073/pnas.090020497

Figure 6.

Figure 6

Kielin has a mesoderm-dorsalizing activity in VMZ assays, but does not induce neural differentiation in the animal cap. VMZ explants were prepared from embryos injected with control frame-shift Kielin (control) mRNA (300 pg; A, D, G, and J), wild-type Kielin (KLN) mRNA (300 pg; B, E, H, and K), and Chd mRNA (100 pg; C, F, I, and L). As Kilein mRNA is three times larger in size than Chd mRNA, the injected mounts of mRNAs are similar in terms of molar ratio. (A–I) VMZ assays. The VMZ explants harvested at stage 13 were analyzed by in situ hybridization with a MyoD probe (A–C), Xnot probe (D–F), or a HNF-3β probe (G–I). (J–L) Animal cap assays. Animal caps were explanted at stage 10.25 from embryos injected with control frame-shift Kielin mRNA (J), wild-type Kielin mRNA (K), and Chd mRNA (L). The animal cap explants were harvested at stage 20 and hybridized with a NCAM probe.