Figure 3. Definition of cell phenotypes in infected lungs and MdLNs.
Single cell suspensions of MdLN (A), washed lung (B), and BALF (C) were prepared from organs collected 48 hrs post infection with 105 CFU of LVS. Cells derived from non-infected animals served as controls. MdLN and BALF cell suspensions were stained for expression of CD11c and CD11b using antibodies conjugated to APC or PE, and analyzed by flow cytometry for surface display of markers as well as autofluorescence at FL1. Data are presented in the form of dot diagrams using parallel gating for identification of infection-triggered recruitments (numbers indicate percentage representations of the gated populations). Isotype-matched antibodies served as controls, and the same gates used in specific stain analysis were evaluated. In this case, gated population frequencies in infected organs were comparable to backgrounds (not shown). All analyses presented were conducted on tissues pooled from 3 individual animals. Three independent experiments exhibited similar cell profiles.