Figure 8. The effects of FTY720 on accumulation of RTDC and LVS in the MdLN.

Mice infected with 10⁵ LVS were treated with FTY720 diluted in carrier solution, or with carrier solution alone, as described in Materials and Methods. To examine DC trafficking, MdLNs were collected 48 hrs post infection and analyzed for RTDC representation (CD11b^high/autofluorescence^low) as described in the legend to Figure 3. An experiment was conducted on MdLN pools, each consisting of 6 organs. MdLNs were derived from infected mice treated with either carrier alone (A) or with FTY720 (B). Non-infected, FTY720-treated mice (C) served as controls. Bacterial colonization of the MdLN (D) was examined 48 hrs post infection in each one of the 6 individual FTY720- treated and 6 mock-treated mice, P<0.01. Three independent experiments, each conducted with groups of six mice, exhibited similar correlation between RTDC and LVS recruitment.