Figure 9. The effects of BW245C on the outcome of LVS infection.

Mice infected with 10⁵ LVS were treated with BW245 diluted in carrier, or with carrier alone, as described in Materials and Methods. To examine DC trafficking (A), MdLNs were collected 48 hrs post infection and analyzed for RTDC representation (CD11b^high/autofluorescence^low). Experiments were conducted on MdLN pools, each consisting of 6 organs, derived from infected or non-infected mice treated with either BW245C or carrier alone. Results are presented by bar diagrams as the averages of three independent experiments. * P<0.05. Bacterial colonization of the MdLN (B) was examined 48 hrs post infection in 13 individual BW245C-treated and 13 mock-treated mice. ** P<0.05. The effect of BW245 on mortality (C) was examined in groups of 10 mice comparing treated (open squares) and mock-treated mice (closed circles). The experiment presented represents one of three similar experiments. The effect of the drug on morbidity (D) was examined by monitoring weight loss for 5 consecutive days in groups of 10 mice each (8-week-old females). Groups consisted of non-infected BW245-treated mice (closed triangles), infected and treated mice (open squares), and infected mock-treated mice (closed circles). Data are presented as percentage of the weight determined on day zero and calculated separately for each individual mouse. *** P-values on days 2–5 range between <0.01 and <0.05.