. Author manuscript; available in PMC: 2009 Sep 23.

Published in final edited form as: Biochemistry. 2008 Aug 29;47(38):10084–10098. doi: 10.1021/bi8003928

Table 1.

Molecular weights and experimental and calculated molar extinction coefficients for the protein constructs used in binding studies.

Protein	Abbn.	Mol. Wt.	E_{280 nm} (cm⁻¹·M⁻¹)

			Experimental	Calculated
NHERF1 PDZ1	N1P1	17555	2464	2980
NHERF1 PDZ2	N1P2	21128	1599	1490
NHERF2 PDZ1	N2P1	19592	12615	12490
NHERF2 PDZ2	N2P2	17672	3105	2980
CAL PDZ	CALP	12689	3034	2980
Full-length CAL	CAL	49875	29583	24410

Molecular weights and molar extinction coefficients (E_280nm) were calculated for each of the constructs used in binding studies. Experimental E_280nm values were determined by measurement of absorbance values for a stock solution that was also subjected to quantitative amino-acid analysis.