Table 3.

K_i values for CAL binding peptides.

Binding partnera	Sequence	Ki (μM)
C-SSR510	CANGLMQTSKL	37.6 ± 4.0
C-AIC3A10	CHRTCYLVTQL	42.2 ± 4.2
C-FRZ810	CYPKQMPLSQV	47 ± 10
C-GBG410	CREKKFFSTILb	63 ± 15
C-CL110	CDGQMQLVTSL	88.3 ± 6.3
C-ClC3B10	CEVYLLNSTTL	93.0 ± 8.5
C-NLG210	CLPHPHSTTRV	106.7 ± 1.7
C-SDK210	CAPIAGFSSFV	150 ± 20
C-NLG10	CHPHSHSTTRV	158 ± 19
C-RTKN10	CQPRTWLQSPV	229 ± 16
C-FRZ510	CYHKQVSLSHV	451 ± 42
C-β1AR10	CRPGFASESKV	510 ± 110c
C-GluRδ210	CGNDPDRGTSI	560 ± 130
C-CFTR10	CTEEEVQDTRL	690 ± 120
C-GluRδ110	CALDTSHGTSI	875 ± 56
C-βCAT10	CNQLAWFDTDL	1110 ± 480d
C-NR2A10	CKKMPSIESDV	1940 ± 920d
C-NGC10	CDVNSLQNNLT	n.b.d.e

K_i values were determined by titration of the indicated peptide sequences in the presence of 30 nM F*C-SSR5₁₀ and 5 μM CALP and determining the fluorescence anisotropy of the reporter ligand. Data were fit using equation 5. Bold entries reflect binding partners identified only on the basis of yeast two-hybrid data.

^aFull names, accession numbers, and references are presented in Supplementary Table S1.

^bThe native sequence contains a Cys at position 8, instead of a Ser.

^cPreviously reported value: 0.035 μM (19).

^dLarge uncertainty reflects substantially incomplete displacement at highest concentration tested.

^en.b.d.: no binding detected.