Table 5. Sample 8 genes with transcript levels at least 2-fold greater than those in in vitro 3D7 samples.
| Gene | Description | Expression units | |||||||
|---|---|---|---|---|---|---|---|---|---|
| In vivo | In vitro 3D7 | ||||||||
| 6 | 7 | 8 | 12 | 29 | M | ER | LR | ||
| Surface proteins | |||||||||
| PFE0005w | PfEMP1 | 114 | 209 | 234 | 184 | 90 | 34 | 95 | 61 |
| PF08_0141 | PfEMP1 | 0 | 90 | 180 | 0 | 0 | 0 | 32 | 0 |
| MAL6P1.316 | PfEMP1 | 0 | 106 | 141 | 0 | 0 | 0 | 32 | 21 |
| PF10_0399 | RIFIN | 197 | 356 | 303 | 185 | 58 | 52 | 0 | 32 |
| PF14_0768 | RIFIN | 162 | 290 | 295 | 182 | 144 | 36 | 39 | 28 |
| PFL0015c | RIFIN | 104 | 443 | 196 | 162 | 71 | 41 | 0 | 28 |
| PFL2660w | RIFIN | 0 | 451 | 177 | 0 | 120 | 40 | 42 | 0 |
| PF11_0515 | RIFIN | 0 | 396 | 174 | 116 | 0 | 0 | 0 | 28 |
| PFI0030c | RIFIN | 124 | 185 | 156 | 120 | 53 | 33 | 16 | 21 |
| PFD0055w | RIFIN | 46 | 138 | 137 | 108 | 38 | 17 | 0 | 13 |
| PF14_0004 | RIFIN | 77 | 164 | 127 | 77 | 48 | 18 | 0 | 17 |
| PFB1010w | RIFIN | 45 | 121 | 115 | 65 | 0 | 19 | 0 | 0 |
| PF13_0007 | RIFIN | 58 | 123 | 106 | 64 | 43 | 0 | 14 | 21 |
| PFA0050c | RIFIN | 0 | 0 | 86 | 96 | 0 | 0 | 0 | 0 |
| PFE0025c | RIFIN | 0 | 0 | 83 | 73 | 0 | 0 | 0 | 0 |
| PFD0130c | RIF pseudogene | 0 | 176 | 77 | 76 | 0 | 30 | 0 | 0 |
| PF10_0394 | RIFIN | 0 | 201 | 45 | 0 | 0 | 0 | 0 | 0 |
| PFA0090c | STEVOR | 197 | 263 | 364 | 245 | 0 | 52 | 36 | 0 |
| PF14_0007 | STEVOR, putative | 0 | 200 | 216 | 108 | 81 | 42 | 0 | 30 |
| PFD0035c | STEVOR | 57 | 502 | 197 | 189 | 0 | 50 | 0 | 0 |
| PFB0955w | STEVOR, degenerate, putative | 0 | 450 | 174 | 217 | 0 | 25 | 19 | 0 |
| PFB0025c | STEVOR, putative | 0 | 276 | 147 | 0 | 0 | 64 | 0 | 56 |
| PF11_0512 | RESA-2 | 530 | 156 | 379 | 443 | 626 | 0 | 0 | 0 |
| Metabolic/cellular function | |||||||||
| PF13_0045 | 40S ribosomal protein S27, putative | 925 | 511 | 1133 | 1248 | 1431 | 0 | 342 | 483 |
| PF14_0183 | RNA helicase, putative | 480 | 131 | 413 | 1373 | 227 | 92 | 154 | 128 |
| PFC0855w | Ubiquitin-conjugating enzyme, putative | 277 | 709 | 396 | 473 | 194 | 0 | 121 | 153 |
| PFC0050c | Long-chain fatty acid ligase, putative | 617 | 186 | 192 | 73 | 92 | 0 | 0 | 0 |
| PFE1265w | G-protein coupled receptor, putative | 40 | 405 | 135 | 91 | 0 | 0 | 0 | 0 |
| PFI1110w | Glutamate-ammonia ligase (glutamine synthetase) | 79 | 289 | 126 | 109 | 66 | 0 | 52 | 37 |
| PF14_0751 | Fatty acyl coenzyme A synthetase-1, putative | 0 | 143 | 80 | 0 | 0 | 0 | 0 | 0 |
| PFI0255c | Mitochondrial carrier protein, putative | 0 | 161 | 62 | 112 | 0 | 0 | 0 | 0 |
| PFI0415c | Ribosomal RNA methyltransferase, putative | 23 | 0 | 42 | 22 | 0 | 0 | 0 | 0 |
| MAL8P1.22 | Dehydrodolichyl diphosphate synthetase, putative | 26 | 130 | 20 | 0 | 14 | 0 | 0 | 0 |
| MAL7P1.92 | Cysteine repeat modular protein 2 homologue | 0 | 0 | 13 | 0 | 0 | 0 | 0 | 0 |
| MAL7P1.164 | Adaptor-related protein, putative | 0 | 0 | 10 | 18 | 0 | 0 | 0 | 0 |
| Hypothetical protein | |||||||||
| PF11_0032 | Hypothetical protein | 1646 | 3627 | 3499 | 2583 | 1071 | 774 | 393 | 697 |
| PFA0620c | Hypothetical protein | 569 | 211 | 2950 | 122 | 1383 | 0 | 913 | 444 |
| MAL7P1.39 | Hypothetical protein | 114 | 2246 | 604 | 504 | 226 | 175 | 105 | 94 |
| PFA0135w | Hypothetical protein | 469 | 313 | 577 | 883 | 307 | 229 | 0 | 37 |
| PF11_0033 | Hypothetical protein | 284 | 1926 | 550 | 544 | 241 | 212 | 49 | 68 |
| chr1.rRNA-1-ITS2 | Hypothetical protein | 147 | 1021 | 395 | 333 | 96 | 49 | 0 | 0 |
| PF14_0752 | Hypothetical protein | 837 | 0 | 314 | 569 | 351 | 44 | 76 | 23 |
| PFA0075w | Hypothetical protein | 0 | 664 | 259 | 195 | 0 | 67 | 0 | 0 |
| PFD0065w | Hypothetical protein | 94 | 276 | 233 | 165 | 285 | 31 | 0 | 0 |
| PF10_0383 | Hypothetical protein, conserved | 111 | 1063 | 229 | 345 | 120 | 105 | 17 | 33 |
| MAL8P1.30 | Hypothetical protein | 118 | 639 | 227 | 206 | 109 | 52 | 28 | 42 |
| PF14_0743 | Hypothetical protein | 119 | 730 | 224 | 174 | 84 | 96 | 0 | 78 |
| PFL0720w | Hypothetical protein | 76 | 672 | 201 | 175 | 86 | 69 | 0 | 38 |
| PFA0245w | Hypothetical protein | 52 | 626 | 170 | 197 | 68 | 39 | 0 | 0 |
| PF14_0298 | Hypothetical protein | 96 | 215 | 163 | 122 | 0 | 0 | 22 | 0 |
| PFL1225c | Hypothetical protein | 0 | 0 | 158 | 0 | 0 | 61 | 0 | 58 |
| PF11_0271 | Hypothetical protein, conserved | 0 | 360 | 146 | 139 | 58 | 0 | 0 | 71 |
| PF11_0006 | Hypothetical protein | 173 | 1188 | 142 | 295 | 178 | 0 | 0 | 0 |
| PF14_0562 | Hypothetical protein | 0 | 509 | 135 | 104 | 0 | 0 | 0 | 24 |
| PF14_0539 | Hypothetical protein | 43 | 385 | 133 | 129 | 49 | 37 | 26 | 0 |
| PFC0505c | Hypothetical protein | 76 | 536 | 130 | 169 | 74 | 43 | 30 | 48 |
| PFL0955c | Hypothetical protein | 0 | 200 | 128 | 102 | 0 | 0 | 0 | 0 |
| PFE0030c | Hypothetical protein | 0 | 238 | 114 | 100 | 0 | 18 | 0 | 0 |
| PF13_0230 | Hypothetical protein | 61 | 141 | 100 | 0 | 0 | 0 | 0 | 0 |
| PFB0950w | Hypothetical protein | 0 | 289 | 100 | 99 | 0 | 0 | 0 | 0 |
| PFI0485c | Hypothetical protein | 55 | 289 | 98 | 144 | 0 | 0 | 0 | 0 |
| PF11_0004 | Hypothetical protein | 49 | 253 | 97 | 152 | 67 | 35 | 16 | 0 |
| PF11_0089 | Hypothetical protein | 0 | 116 | 95 | 0 | 46 | 12 | 0 | 0 |
| PFL1660c | Hypothetical protein | 0 | 253 | 90 | 48 | 0 | 23 | 0 | 20 |
| PFA0720w | Hypothetical protein | 31 | 365 | 86 | 116 | 0 | 0 | 0 | 0 |
| PFE0095c | Hypothetical protein | 0 | 0 | 70 | 0 | 0 | 0 | 0 | 0 |
| MAL7P1.52 | Hypothetical protein | 0 | 258 | 63 | 92 | 0 | 0 | 0 | 0 |
| MAL6P1.233 | Hypothetical protein | 0 | 0 | 54 | 0 | 0 | 0 | 0 | 0 |
| PF11_0411 | Hypothetical protein | 11 | 163 | 45 | 0 | 0 | 0 | 0 | 0 |
| PF14_0158 | Hypothetical protein | 0 | 205 | 44 | 0 | 0 | 0 | 0 | 0 |
| PF13_0151 | Hypothetical protein | 0 | 0 | 43 | 0 | 0 | 0 | 0 | 0 |
| mitopl_rpoC | Hypothetical protein | 0 | 0 | 38 | 0 | 0 | 0 | 0 | 0 |
| PFB0395w | Hypothetical protein | 0 | 0 | 37 | 0 | 0 | 0 | 0 | 0 |
| PF07_0109 | Hypothetical protein | 0 | 0 | 34 | 0 | 0 | 0 | 0 | 0 |
| PFB0690w | Hypothetical protein | 0 | 0 | 34 | 0 | 0 | 10 | 0 | 0 |
| MAL7P1.89 | Hypothetical protein | 0 | 0 | 30 | 22 | 0 | 0 | 0 | 0 |
| MAL13P1.179 | Hypothetical protein | 0 | 53 | 28 | 0 | 0 | 0 | 0 | 0 |
| PF14_0558 | Hypothetical protein | 19 | 0 | 27 | 0 | 0 | 0 | 0 | 0 |
| PFL2525c | Hypothetical protein | 38 | 182 | 25 | 88 | 0 | 0 | 0 | 0 |
| PF08_0013 | Hypothetical protein | 14 | 0 | 22 | 0 | 0 | 0 | 0 | 0 |
| PFB0110w | Hypothetical protein | 0 | 0 | 20 | 0 | 0 | 0 | 0 | 0 |
| PF11_0421 | Hypothetical protein | 0 | 0 | 19 | 15 | 0 | 0 | 0 | 0 |
| PFI1785w | Hypothetical protein | 0 | 0 | 18 | 0 | 0 | 0 | 0 | 0 |
| PF13_0012 | Hypothetical protein | 0 | 0 | 18 | 0 | 0 | 0 | 0 | 0 |
| PF14_0521 | Hypothetical protein | 11 | 0 | 18 | 0 | 0 | 0 | 0 | 0 |
| PF13_0038 | Hypothetical protein | 0 | 95 | 18 | 0 | 0 | 0 | 0 | 0 |
| PF14_0202 | Hypothetical protein | 0 | 0 | 17 | 0 | 0 | 0 | 0 | 0 |
| PFE0190c | Hypothetical protein | 0 | 0 | 16 | 0 | 0 | 0 | 0 | 0 |
| PF10_0206 | Hypothetical protein | 0 | 0 | 16 | 0 | 0 | 0 | 0 | 0 |
| PFC0075c | Hypothetical protein | 18 | 0 | 16 | 32 | 0 | 0 | 0 | 0 |
| MAL13P1.32 | Hypothetical protein | 0 | 0 | 16 | 0 | 0 | 0 | 0 | 0 |
| PFL2385c | Hypothetical protein | 0 | 0 | 15 | 20 | 0 | 0 | 0 | 0 |
| PF08_0070 | Hypothetical protein | 0 | 0 | 15 | 14 | 0 | 0 | 0 | 0 |
| PFB0960c | Hypothetical protein | 0 | 0 | 15 | 0 | 0 | 0 | 0 | 0 |
| PF11_0410 | Hypothetical protein | 0 | 0 | 13 | 11 | 0 | 0 | 0 | 0 |
| PF14_0049 | Hypothetical protein | 0 | 0 | 13 | 0 | 0 | 0 | 0 | 0 |
| PF11_0064 | Hypothetical protein | 0 | 0 | 13 | 0 | 0 | 0 | 0 | 0 |
| PF14_0116 | Hypothetical protein | 16 | 19 | 13 | 12 | 15 | 0 | 0 | 0 |
| PFI0815c | Hypothetical protein, conserved | 0 | 0 | 13 | 0 | 0 | 0 | 0 | 0 |
| PF10_0055 | Hypothetical protein | 0 | 0 | 12 | 0 | 0 | 0 | 0 | 0 |
| PFC0215c | Hypothetical protein | 0 | 0 | 12 | 0 | 0 | 0 | 0 | 0 |
| PFA0385w | Hypothetical protein | 0 | 121 | 12 | 0 | 0 | 0 | 0 | 0 |
| PFB0946c | Hypothetical protein | 0 | 0 | 12 | 0 | 0 | 0 | 0 | 0 |
| MAL6P1.187 | Hypothetical protein | 0 | 0 | 12 | 0 | 0 | 0 | 0 | 0 |
| PFE0610c | Hypothetical protein | 0 | 0 | 12 | 13 | 0 | 0 | 0 | 0 |
| PF10_0158 | Hypothetical protein | 0 | 0 | 12 | 0 | 0 | 0 | 0 | 0 |
| PFE0085c | Hypothetical protein | 0 | 0 | 11 | 0 | 0 | 0 | 0 | 0 |
| MAL8P1.41 | Hypothetical protein | 15 | 0 | 11 | 0 | 0 | 0 | 0 | 0 |
| MAL6P1.166 | Hypothetical protein | 0 | 11 | 11 | 0 | 0 | 0 | 0 | 0 |
| PF13_0075 | Hypothetical protein | 0 | 17 | 11 | 0 | 0 | 0 | 0 | 0 |
| PFB0510w | Hypothetical protein | 0 | 0 | 11 | 23 | 0 | 0 | 0 | 0 |
| PF14_0534 | Hypothetical protein | 0 | 0 | 11 | 0 | 0 | 0 | 0 | 0 |
| PFE1180c | Hypothetical protein | 0 | 0 | 11 | 10 | 0 | 0 | 0 | 0 |
| PFL0985c | Hypothetical protein | 0 | 24 | 11 | 0 | 12 | 0 | 0 | 0 |
| PFL2020c | Hypothetical protein | 0 | 0 | 11 | 0 | 19 | 0 | 0 | 0 |
| PFI1305w | Hypothetical protein | 0 | 0 | 11 | 0 | 0 | 0 | 0 | 0 |
| PF11_0462 | Hypothetical protein | 0 | 0 | 11 | 0 | 0 | 0 | 0 | 0 |
| PFB0470w | Hypothetical protein | 18 | 0 | 10 | 0 | 0 | 0 | 0 | 0 |
| PFD0870w | Hypothetical protein | 0 | 0 | 10 | 0 | 0 | 0 | 0 | 0 |
| PFC0130c | Hypothetical protein | 0 | 0 | 10 | 0 | 0 | 0 | 0 | 0 |
NOTE. ER, early ring; LR, late ring; M, merozoite; PfEMP1, erythrocyte membrane protein 1