Table 3.
Real-time RT-PCR results for RNA isolated from faecal samples using different methods of RNA extraction
| FIPV 79–1146 | Faeces 14 | Faeces 15 | Faeces 16 | Faeces 17 | Media control | RNA water control | PCR water control | |
|---|---|---|---|---|---|---|---|---|
| Boom extraction method | 36.4 | 34.9 | 40.5 | 34.1 | 36.2 | (−) | (−) | (−) |
| QIAgen viral RNA mini kit | 20.2 | 35.0 | 34.5 | 31.8 | 32.8 | (−) | (−) | (−) |
| QIAgen stool DNA mini kit | 21.1 | 38.1 | 37.4 | 33.3 | 34.0 | (−) | (−) | (−) |
Results are expressed as threshold cycle (CT) values and positive (FIPV 79–1146) and negative (media and water) controls are included. Negative results are represented as (−).