Table 2.
Clinical Response* | Histological Response Assessment (Cases) | Biochemistry | Immunohistochemistry† | ||||||||||||||||||
Residual Cellularity Assessment (Frozen Samples)‡ | WB Beclin1 | WB PI3KIII | WB Bcl2 | WB LC3B-II | Co-IP Experiments | WB Caspase 3 | WB Caspase 7 | WB Lamin A | Beclin1 | PI3KIII | Bcl2 | Caspase 3 | Lamin A | ||||||||
IP Beclin1/WB Beclin1 | IP Beclin1/WB PI3KIII | IP Beclin1/WB Bcl2 | Full Length | Cleaved | Cleaved | Full Length | Cleaved | Cleaved | Full Length | Cleaved | |||||||||||
Treated patients§ | |||||||||||||||||||||
1 | r | High | b | 1+ | +w | +w | - | 1+ | ne | - | +w | - | - | 1+ | - | + | +w | +w | - | + | - |
2 | r | High | b | 1+ | +w | +w | - | 1+ | +w | - | 1+ | - | - | 1+ | - | + | + | + | - | + | - |
3 | r | High | b | 1+ | +w | +w | +w | 1+ | 1+ | - | 1+ | - | - | 1+ | - | + | +w | +w | - | + | - |
4 | r | Moderate | c | 2+ | 1+ | 1+ | +w | 2+ | 1+ | +w | 1+ | - | - | 1+ | - | + | + | + | - | + | - |
5 | r | Moderate | c | 2+ | 1+ | 1+ | 1+ | 2+ | 1+ | +w | 1+ | - | - | 1+ | - | + | + | +w | - | + | - |
6 | r | Moderate | c | 2+ | +w | +w | +w | 2+ | 1+ | +w | 1+ | - | - | 1+ | - | + | + | +w | - | + | - |
7 | r | Low | a | 1+ | - | - | - | 1+ | - | - | 1+ | - | - | 1+ | - | + | + | + | - | + | - |
8 | r | Low | d | 2+ | 2+ | 1+ | 2+ | 2+ | 2+ | 1+ | 1+ | - | - | 1+ | - | + | + | +w | - | + | - |
9 | r | Low | d | 2+ | 2+ | 2+ | 2+ | 2+ | 1+ | 1+ | 1+ | - | - | 1+ | - | + | + | + | - | + | - |
10 | p | Low | d | 2+ | 2+ | 2+ | 2+ | 2+ | 1+ | +w | 1+ | - | - | 1+ | - | + | + | + | - | + | - |
11 | p | Low | d | 2+ | 1+ | 1+ | +w | 2+ | 1+ | +w | 1+ | - | - | 1+ | - | + | + | + | - | + | - |
Untreated patients | |||||||||||||||||||||
1 | / | 2+ | 2+ | 1+ | 2+ | 2+ | 1+ | +w | 1+ | - | - | 1+ | - | + | + | + | - | + | - | ||
2 | / | 2+ | 2+ | 2+ | 2+ | 2+ | 2+ | 1+ | 1+ | - | - | 1+ | - | + | + | ++ | - | + | - |
+w indicates weak positive; Co-IP, coimmunoprecipitation; IP, immunoprecipitation; ne, not evaluable.
p indicates progression; r, response.
Immunohistochemistry of paraffin-embedded sections of samples showing comparable residual cellularity to that of the corresponding frozen samples.
a <10%, b 10% to 50%, c 50% to 90%, d >90%.
The numbers in bold are patients who participated in a study of advanced GIST (three of which, nos. 4, 6, and 7, were described in a previous article [3] as 14, 19, and 25, respectively); the numbers in italics are those treated using the neoadjuvant protocol.