Table 4. Position specific score matrix (PSSM) analysis of the predicted p.G948W substitution in EPHA2.
|
Codon |
Amino acid |
Side-chain |
Charge |
Potential H-bonds |
Residue weight |
Isoelectric point |
Hydro-phobicity |
PSSM value |
|---|---|---|---|---|---|---|---|---|
| GGG |
G (Gly) |
-H |
0 |
0 |
57 |
6.0 |
0.770 |
6 |
| TGG | W (Trp) | -CH2-C8 H4NH | 0 | 1 | 186 | 5.9 | 0.854 | −6 |
Differences in the physico-chemical properties of glycine and tryptophan are consistent with a non-conservative substitution. The negative PSSM value indicates that tryptophan substitutes for glycine at position 948 less frequently than expected among proteins containing the conserved SAM domain.