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RT-PCRs for bone morphogenetic protein-4 (BMP-4; upper panel) of adrenal explant cultures of a developmental stage S23 chick embryo after 4 days in vitro (lane a). Levels of BMP-4 mRNA are comparable to those in vivo at S23 (lane b) and S32 (lane c). RT-PCRs of GAPDH (lower panel) were run in parallel.
