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. 2008 Oct 28;5:130. doi: 10.1186/1743-422X-5-130

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Copyright © 2008 Lim et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analysis of HA-FO and H5-VLP. (A) The indicated HA foldons from recombinant baculovirus were collected and allowed to incubate with Talon resin. Following elution, equal amounts were treated with trypsin to check for correct folding, heat denaturation was used as a control for trypsin activity. Proteins were resolved on 12% SDS-PAGE and stained with coomassie. Mw markers (sizes in kDa, left) and the bands representing HA0, HA1 and HA2 are indicated. (B) H5-VLP were released from HEK293 cells by addition of exogenous NA, the VLP pellet after sucrose cushion purification was resolved on 12% SDS-PAGE. Immunoblots were probed with α-HA (VN04-2).