Table 4.
MW (kDa)a | Probable T7 protein | aa sequence | Identity (%) | BLASTp similarity (phage and accession nr.) | Alignment |
137.3 | |||||
98.4 | |||||
85.6 | |||||
74.5 | |||||
68.5 | |||||
63.1 (p1) | Tail fiber (monomeric form of gp17)1 | KEVLFGDS | 80 | Hyp. protein (phage BA3, YP001552271) | KEVLFG – DS KEVLFG – DS KEVLFGLNDS |
59.7 (p2) | Head-tail connector (gp8)1 | ||||
50.5 | - | No sequence | - | N-terminally blocked | - |
45.2 (p3) | Major capsid (gp10A)2 | AQMQGGQQIG | 80 | Major capsid protein 10A (phage ϕYeO3-12, NP_052109; phage T3, NP_523335) | AQMQGGQQIG A – QGGQQIG ANIQGGQQIG |
39.3 | |||||
38.2 | |||||
30.6 (p4) | Capsid assembly (gp9)1 | ||||
29.0 | |||||
25.9 | |||||
18.6 (p5) | Internal virion B (gp14)1 | ||||
16.6 |
Molecular weights of phage ϕIBB-PF7A structural proteins were determined from SDS-PAGE gels and compared with known T7 structural proteins with approximate weights, N-terminal sequence of proteins, similarities and respective accession numbers
a) Average from 2 separate determinations; 1 Kovalyova and Kropinski, 2003; 2 Pajunen et al. 2000.