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. 2008 Oct 27;8:80. doi: 10.1186/1472-6750-8-80

Table 4.

Phage ϕIBB-PF7A structural proteins

MW (kDa)a Probable T7 protein aa sequence Identity (%) BLASTp similarity (phage and accession nr.) Alignment
137.3

98.4

85.6

74.5

68.5

63.1 (p1) Tail fiber (monomeric form of gp17)1 KEVLFGDS 80 Hyp. protein (phage BA3, YP001552271) KEVLFG – DS
KEVLFG – DS
KEVLFGLNDS

59.7 (p2) Head-tail connector (gp8)1

50.5 - No sequence - N-terminally blocked -

45.2 (p3) Major capsid (gp10A)2 AQMQGGQQIG 80 Major capsid protein 10A (phage ϕYeO3-12, NP_052109; phage T3, NP_523335) AQMQGGQQIG
A – QGGQQIG
ANIQGGQQIG

39.3

38.2

30.6 (p4) Capsid assembly (gp9)1

29.0

25.9

18.6 (p5) Internal virion B (gp14)1

16.6

Molecular weights of phage ϕIBB-PF7A structural proteins were determined from SDS-PAGE gels and compared with known T7 structural proteins with approximate weights, N-terminal sequence of proteins, similarities and respective accession numbers

a) Average from 2 separate determinations; 1 Kovalyova and Kropinski, 2003; 2 Pajunen et al. 2000.