Fig. 5.

Impact of fasudil on vitreous-induced MLC phosphorylation. After pretreatment with or without anti-TGF-β mAb or fasudil, hyalocytes were stimulated with recombinant TGF-β2 (A), vitreous with PDR (B), or vitreous with PVR (C) for 24 h (n = 3, each). Western blot analysis was performed to detect phosphorylated MLC (pMLC). Lane-loading differences were normalized by MLC. Signal intensities were quantified and expressed as percentages of the pMLC/MLC ratio compared with control (treated with DMEM). *, P < 0.05; **, P < 0.01.