Fig. 4.

Movement of individual cross-bridges in response to ATP application. (A) Comparison of the cross-bridge position between the 2 IP records, taken before and after ATP application. Open and filled circles (diameter, 20 nm) are drawn around the center of mass positions of the same particles before and after ATP application, respectively. (Inset) An example of superimposed IP records showing the change in position of the same particle, which are colored red (before ATP application) and blue (after ATP application). The center of mass position for each particle image is located at the center of the circle on the particle image. (B) Histogram showing distribution of the amplitude of ATP-induced cross-bridge movement, obtained from the ATP-induced changes in the center of mass position of each gold particle.