Fig. 6.

Examples showing sequential changes in position of different pixels (each 2.5 × 2.5 nm) where the center of mass positions of corresponding different particles are located. In each frame, the pixel positions were recorded 3 times, i.e., before ATP application, during ATP application, and after exhaustion of applied ATP. The changes in position of the pixels in the first (red), second (blue), and third (yellow) records indicate changes in the cross-bridge position before ATP application, during ATP application, and after exhaustion of ATP. Direction of each cross-bridge movement is indicated by an arrow. The angle between the filament long axis, on which the particles was located, and the X–Y coordinates of the IP was random. Note that the cross-bridges return toward their initial position after exhaustion of ATP. After exhaustion of ATP, the cross bridge returned almost exactly to its initial position in A, B, and I, and close to its initial position in C–H.