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Kinetic evaluation of catalyzed ghrelin hydrolysis. (Left) Catalysis of ghrelin hydrolysis by addition of 1 μM GHR-11E11 to varying concentrations of substrate 3 (0.4–50 μM) at 37 °C. (Right) Dose-response plot of inhibiton of ghrelin hydrolysis by 1 μM GHR-11E11 with varying inhibitor 1 concentrations; IC50: 21.3μM.
