Fig. 3.

Shown are the rate of energy expenditure (heat, Top Left), respiratory exchange ratio (RER) (Top Right), the rates of oxygen consumption (VO₂) (Middle Left) and carbon dioxide production (VCO₂) (Middle Right), and rates of horizontal and vertical motor activity (Bottom) in food-deprived, antibody-treated, adult male C57BL/6J mice tested in open-circuit indirect calorimetry chambers. Data are expressed in 2 h bins as M ± SEM across the 12-h light cycle. Mice received i.v. administration (i.v. 50 mg/kg) of a catalytic antibody against ghrelin (n = 8, GHR-11E11) or of an isotype-matched nicotine control Ab (n = 9, NIC-1 9D9) before data collection; *, P < 0.05 vs. control Ab-treated mice.