Fig. 5.

Panels show the rate of energy expenditure (heat) (Top Left), respiratory exchange ratio (RER) (Top Right), rates of oxygen consumption (VO₂) (Bottom Left), and carbon dioxide production (VCO₂) (Bottom Right) in adult male C57BL/6J mice residing in open-circuit indirect calorimetry chambers. Data were collected in a 24-h food-deprived state (“Unfed”) and during 6 h of refeeding on chow. Data are expressed in 1 h bins as M ± SEM. Mice had received i.v. administration (i.v. 50 mg/kg) of a catalytic antibody against ghrelin (n = 8, GHR-11E11) or of an isotype-matched nicotine control Ab (n = 9, NIC-1 9D9) 24 h before data collection; *, P < 0.05 vs. control Ab-treated mice.