Fig. 2.

Paxillin coredistributes with clustered IgE-FcεRI and patterned lipid bilayers, but integrins avoid these regions. Confocal micrographs show cells expressing paxillin-EGFP or integrin α5-EGFP (green) after incubation for 40 min at 37°C with patterned lipid bilayers (red) containing DNP-cap-PE and fixation before imaging. (A) Paxillin is recruited with IgE-FcεRI in the regions of patterned ligands, and α5-integrin is excluded. (Scale bars: 20 μm.) (B) Intensity line profiles from images in A confirm that paxillin accumulation and integrin exclusion correspond exactly to where the patterned bilayers are localized.