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. 2008 Nov 3;105(45):17402–17407. doi: 10.1073/pnas.0802781105

Fig. 4.

Fig. 4.

Ceramide-induced nutrient transporter loss leads to CICD in the absence of apoptosis. (A) Viability of control or Bcl-XL-expressing FL5.12 cells following growth factor withdrawal. (B) Control or Bcl-XL-expressing cells were treated with 25 μM C2-cer with or without MP supplementation. (C) FL5.12 cells were withdrawn from IL-3 in the presence or absence of 50 μM zVAD. (D) The indicated cells were treated with 25 μM C2-cer with or without zVAD. (E) Nuclear morphology of FL5.12 cells maintained +/−IL-3 for 17 h or Bcl-XL-expressing cells treated with 25 μM C2-cer for 24 h. PI is a vital dye, Hoechst 33342 stains DNA in live cells. Scale bar, 10 μm. (F) Apoptosis was measured in control FL5.12 cells maintained +/−IL-3 for 9 h or in Bcl-XL-expressing cells treated with 25 μM C2-cer for 18 h. Error bars, SD.