Figure 5. Activation of the mammalian target of rapamycin (mTOR) pathway in adiponectin-deficient (KO) mice compared with that in wild-type (WT) mice under the high-fat diet condition. Western blot analysis for phosphorylated and total AMP-activated protein kinase (AMPK) (A), mTOR (B), p70 S6 kinase (C) and S6 protein (D) in the colon from the WT and KO mice under the high-fat diet condition. Left panels: Pictures of the western blotting. Right panels: Graphs showing the ratios of the phosphorylated protein to the total protein. Each column represents the mean (with the SEM), *p<0.05.