Fig. 7.

The dependence of AA concentrations on the formation of several quantitatively predominant AA metabolites (PGF_2α, PGE₂, PGD₂, and 12-HHT) when 100 μM myricetin of present (filled circle) or absence (open circle). The incubation mixture consisted of indicated [¹⁴C]AA (0.2 μCi) as substrate, COX I or COX II as the enzyme (0.5 or 0.97 μg/ml, respectively), 10 mM EDTA, 1 mM reduced glutathione, 1 μM hematin, and with (filled circle) or without (open circle) 100 μM myricetin in 200 μl of 100 mM Tris-HCl buffer, pH 7.4. The incubations were carried out at 37°C for 5 min. Each data point is the mean of duplicate determinations. The kinetic paramters (K_m, V_max and V_max/K_m) for all AA metabolites were summarized in Table 1.