TABLE 1.

Kinetic parameters (K_m and V_MAX values) for the COX-mediated formation of PGF_2α, PGE₂, PGD₂, and 12-hydroxy-5Z,8E,10E-heptadecatrienoic acid (12-HHT)

		COX II			COX I
Product	Myricetin	Km (μM)	Vmax (nmol/μg/5 min)	Vmax/Km	Km (μM)	Vmax (nmol/μg/5 min)	Vmax/Km
PGF2α	0 μM	1.8	38	20.8	4.0	23	5.8
	100 μM	9.9	236	23.8	4.5	90	20
PGE2	0 μM	1.2	41	34.2	1.9	29	15.3
	100 μM	5.5	457	83.0	2.2	160	72.7
PGD2	0 μM	0.9	30	33.3	5.8	19	3.3
	100 μM	2.4	179	74.6	5.3	72	13.6
12-HHT	0 μM	1.0	44	43.5	1.5	26	17.3
	100 μM	2.6	375	144.0	1.8	190	105.6
5-HETE	0 μM	4.2	7	1.7	4.2	4	1
	100 μM	13	7	0.5	6.4	2	0.3
11-HETE	0 μM	9.7	14	1.4	4.6	5	1.1
	100 μM	15.4	142	9.2	4.8	35.2	7.3
12-HETE	0 μM	8.4	11	1.3	5	13	2.6
	100 μM	15.6	7	0.4	6.6	2	0.3
15-HETE	0 μM	8.2	16	2.0	261.3	222	0.8
	100 μM	35.2	168	4.8	126.1	230	1.8

COX, cyclooxygenase; HETE, hydroxyeicosatetraenoic acid.