TABLE 1.
Mass content of cardiolipin molecular species of mitochondria isolated from brain and brain tumors
| [M-2H]- | Molecular Species | B6 Brain | CT-2A | EPEN | VM Brain | VM NM1 | VM M2 | VM M3 |
|---|---|---|---|---|---|---|---|---|
| 684.95 | 18:1-16:1-16:1-16:1 | 0.04±0.01 | 0.94±0.31 | |||||
| 685.96 | 18:1-16:1-16:1-16:0 | 1.31±0.03 | 1.24±0.48 | |||||
| 686.97 | 18:1-16:1-16:0-16:0 | 1.06±0.26 | 0.88±0.19 | 1.05±0.30 | ||||
| 698.97 | 18:2-18:1-16:1-16:1 | 1.14±0.12 | 0.17±0.01 | 0.77±0.29 | 0.88±0.02 | 0.92±0.11 | 0.59±0.14 | |
| 699.98 | 18:1-18:1-16:1-16:1 | 0.30±0.03 | 1.33±0.34 | 0.26±0.05 | 0.95±0.14 | 0.63±0.05 | 1.29±0.08 | 0.58±0.12 |
| 700.99 | 18:1-18:1-16:0-16:1 | 0.31±0.05 | 1.08±0.23 | 0.32±0.07 | 0.97±0.35 | 0.51±0.09 | 0.97±0.17 | 0.59±0.21 |
| 18:2-18:1-16:0-16:0 | ||||||||
| 18:2-18:0-16:1-16:0 | ||||||||
| 701.99 | 18:1-18:1-16:0-16:0 | 0.24±0.04 | 0.87±0.17 | 0.44±0.11 | 0.65±0.29 | 0.47±0.03 | 1.14±0.17 | 0.67±0.25 |
| 18:2-18:0-16:0-16:0 | ||||||||
| 18:1-18:0-16:0-16:1 | ||||||||
| 709.96 | 20:4-18:2-16:1-16:1 | 0.09±0.01 | 0.17±0.07 | 1.03±0.39 | ||||
| 710.97 | 20:4-18:1-16:1-16:1 | 0.41±0.06 | 1.25±0.17 | 0.24±0.01 | 2.02±0.12 | |||
| 20:4-18:2-16:1-16:0 | ||||||||
| 711.98 | 20:4-18:1-16:1-16:0 | 0.45±0.06 | 1.62±0.11 | 0.46±0.09 | 0.95±0.28 | 1.91±0.02 | 2.35±0.16 | 1.51±0.23 |
| 18:2-18:2-18:1-16:1 | ||||||||
| 712.99 | 18:2-18:1-18:1-16:1 | 0.71±0.02 | 1.91±0.07 | 0.73±0.17 | 1.62±0.05 | 1.92±0.11 | 1.04±0.15 | 1.00±0.19 |
| 18:1-18:1-18:1-16:2 | ||||||||
| 18:2-18:1-18:0-16:2 | ||||||||
| 18:2-18:2-18:0-16:1 | ||||||||
| 713.99 | 18:1-18:1-18:1-16:1 | 1.47±0.23 | 2.00±0.63 | 0.75±0.15 | 3.34±0.73 | 1.35±0.08 | 1.99±0.31 | 1.33±0.20 |
| 18:2-18:1-18:1-16:0 | ||||||||
| 715.00 | 18:1-18:1-18:1-16:0 | 0.68±0.04 | 1.57±0.30 | 0.39±0.08 | 1.30±0.09 | 0.59±0.08 | 1.34±0.08 | 0.76±0.15 |
| 18:0-18:1-18:1-16:1 | ||||||||
| 716.01 | 18:0-18:1-18:1-16:0 | 0.09±0.02 | 0.17±0.05 | 0.76±0.43 | ||||
| 18:0-18:0-18:1-16:1 | ||||||||
| 721.96 | 20:4-20:4-16:1-16:1 | 0.23±0.03 | 0.27±0.08 | |||||
| 722.97 | 20:4-20:4-16:1-16:0 | 0.36±0.03 | 0.18±0.05 | 0.60±0.10 | ||||
| 20:4-18:2-18:2-16:1 | ||||||||
| 723.98 | 20:4-18:2-18:1-16:1 | 0.95±0.08 | 1.36±0.21 | 0.65±0.14 | 2.01±0.13 | 2.24±0.19 | 1.42±0.32 | 7.42±1.09 |
| 724.99 | 20:4-18:2-18:1-16:0 | 2.22±0.17 | 1.61±0.31 | 1.07±0.19 | 3.08±0.34 | 3.38±0.14 | 1.35±0.11 | 6.07±0.44 |
| 20:4-18:1-18:1-16:1 | ||||||||
| 725.99 | 20:4-18:1-18:1-16:0 | 1.74±0.13 | 2.09±0.04 | 1.42±0.31 | 2.46±0.26 | 3.93±0.29 | 1.58±0.05 | 3.83±0.65 |
| 20:3-18:1-18:1-16:1 | ||||||||
| 727.00 | 18:2-18:1-18:1-18:1 | 2.48±0.33 | 0.77±0.15 | 3.73±0.18 | 1.52±0.13 | 1.35±0.40 | 1.90±0.22 | |
| 728.01 | 18:1-18:1-18:1-18:1 | 3.72±0.72 | 0.32±0.05 | 5.13±0.95 | 0.65±0.09 | 1.33±0.33 | 0.97±0.17 | |
| 729.02 | 18:1-18:1-18:1-18:0 | 0.30±0.03 | 0.11±0.03 | 0.75±0.26 | 0.33±0.06 | |||
| 733.96 | 22:6-20:4-16:1-16:1 | 0.14±0.02 | 0.04±0.01 | |||||
| 734.97 | 20:4-20:4-18:2-16:1 | 0.56±0.06 | 0.07±0.02 | |||||
| 735.98 | 20:4-20:4-18:1-16:1 | 1.41±0.16 | 0.20±0.05 | 2.29±0.67 | 0.63±0.11 | |||
| 736.99 | 20:4-20:4-18:1-16:0 | 2.11±0.07 | 1.00±0.07 | 0.41±0.14 | 2.78±0.42 | 1.18±0.10 | 2.38±0.62 | 1.23±0.26 |
| 22:6-18:1-18:1-16:1 | ||||||||
| 22:6-18:2-18:1-16:0 | ||||||||
| 737.99 | 20:4-18:2-18:1-18:1 | 2.68±0.37 | 1.20±0.28 | 0.46±0.10 | 3.52±0.65 | 1.21±0.25 | 2.32±0.54 | 1.67±0.26 |
| 22:6-18:1-18:1-16:0 | ||||||||
| 739.00 | 20:4-18:1-18:1-18:1 | 3.30±0.45 | 0.39±0.11 | 3.71±0.45 | 0.81±0.08 | 1.27±0.15 | 1.61±0.25 | |
| 740.01 | 20:4-18:1-18:1-18:0 | 1.07±0.14 | 0.19±0.04 | 1.62±0.30 | 0.94±0.38 | 2.08±0.85 | 3.35±0.62 | |
| 20:3-18:1-18:1-18:1 | ||||||||
| 741.02 | 20:4-18:1-18:0-18:0 | 0.25±0.08 | 0.10±0.02 | 2.34±0.30 | ||||
| 20:3-18:1-18:1-18:0 | ||||||||
| 746.97 | 22:6-18:3-18:2-18:2 | 0.40±0.04 | ||||||
| 747.98 | 20:4-20:4-18:2-18:2 | 1.38±0.09 | 0.25±0.04 | 2.81±0.47 | ||||
| 20:4-20:4-20:4-16:0 | ||||||||
| 22:6-20:4-18:1-16:1 | ||||||||
| 22:6-22:6-16:0-16:0 | ||||||||
| 22:6-18:2-18:2-18:2 | ||||||||
| 748.99 | 20:4-20:4-18:2-18:1 | 1.99±0.19 | 0.19±0.03 | |||||
| 749.99 | 20:4-20:4-18:1-18:1 | 3.24±0.24 | 0.26±0.04 | |||||
| 751.00 | 22:6-18:1-18:1-18:1 | 2.74±0.10 | 0.17±0.02 | |||||
| 20:4-20:3-18:1-18:1 | ||||||||
| 752.01 | 22:6-18:1-18:1-18:0 | 0.54±0.17 | 0.12±0.04 | |||||
| 753.02 | 20:4-20:2-18:1-18:0 | 0.15±0.04 | 0.07±0.01 | |||||
| 20:4-20:1-18:1-18:1 | ||||||||
| 754.03 | 20:4-20:1-18:1-18:0 | 0.09±0.02 | 0.05±0.02 | |||||
| 755.03 | 20:4-20:1-18:0-18:0 | 0.04±0.01 | ||||||
| 758.97 | 22:6-20:4-20:4-16:1 | 0.32±0.04 | 0.05±0.02 | 0.89±0.19 | ||||
| 22:6-22:6-18:2-16:1 | ||||||||
| 759.98 | 22:6-20:4-18:2-18:2 | 0.76±0.05 | 0.07±0.03 | |||||
| 22:6-20:4-20:4-16:0 | ||||||||
| 22:6-22:6-18:1-16:1 | ||||||||
| 760.99 | 22:6-20:4-18:2-18:1 | 1.72±0.08 | 0.18±0.08 | 1.31±0.32 | ||||
| 761.99 | 22:6-20:4-18:1-18:1 | 2.94±0.23 | 0.11±0.02 | 1.35±0.15 | 0.97±0.16 | |||
| 763.00 | 22:6-20:4-18:1-18:0 | 1.03±0.05 | 0.07±0.02 | 1.16±0.26 | 1.45±0.22 | |||
| 22:6-20:3-18:1-18:1 | ||||||||
| 764.01 | 22:4-20:4-18:1-18:1 | 0.24±0.02 | 0.11±0.03 | 1.48±0.33 | 1.47±0.24 | |||
| 22:6-20:3-18:1-18:0 | ||||||||
| 765.02 | 22:4-20:4-18:1-18:0 | 0.08±0.01 | ||||||
| 770.97 | 22:6-20:4-20:4-18:3 | 0.18±0.05 | 0.09±0.02 | |||||
| 771.98 | 22:6-22:6-20:4-16:0 | 0.42±0.05 | 0.10±0.04 | 2.04±0.23 | ||||
| 20:4-20:4-20:4-20:4 | ||||||||
| 22:6-20:4-20:4-18:2 | ||||||||
| 22:6-22:6-18:2-18:2 | ||||||||
| 772.99 | 22:6-20:4-20:4-18:1 | 1.42±0.12 | 2.03±0.11 | 0.14±0.04 | 2.65±0.12 | 2.25±0.45 | 2.32±0.31 | |
| 773.99 | 22:6-22:6-18:1-18:1 | 1.81±0.09 | 1.64±0.18 | 0.11±0.02 | 2.68±0.10 | 2.30±0.99 | 2.22±0.42 | |
| 22:6-20:4-20:3-18:1 | ||||||||
| 775.00 | 16:0-20:3-22:5-22:5 | 0.28±0.10 | ||||||
| 18:2-20:3-20:3-22:5 | ||||||||
| 18:0-18:1-22:6-22:6 | ||||||||
| 18:0-20:3-20:4-22:6 | ||||||||
| 776.01 | 18:0-18:0-22:6-22:6 | 0.16±0.05 | ||||||
| 783.98 | 22:6-22:6-20:4-18:2 | 0.33±0.05 | ||||||
| 785.99 | 22:6-22:6-20:4-18:1 | 1.34±0.08 | ||||||
| 22:6-20:4-20:4-20:3 | ||||||||
| 795.98 | 20:4-20:4-22:6-22:6 | 0.25±0.09 | 0.14±0.06 | |||||
| 796.99 | 22:6-22:6-22:6-18:1 | 0.50±0.04 | 0.12±0.05 | |||||
| 22:6-22:6-20:4-20:3 | ||||||||
| 797.99 | 20:4-20:3-22:6-22:5 | 0.16±0.07 | 0.06±0.02 | |||||
| 22:6-22:6-22:6-18:0 | ||||||||
| 22:6-22:6-22:5-18:1 | ||||||||
| 807.98 | 20:4-22:6-22:6-22:6 | 0.07±0.03 | ||||||
| 813.02 | 22:6-22:6-22:6-22:5 | 0.06±0.02 |
Lipid extracts from purified mitochondria were prepared using a modified Bligh and Dyer procedure. The cardiolipin (CL) molecular species in the lipid extracts were identified by searching for plus-one isotopologues of double-charged CL ions followed by product-ion analyses of these plus-one isotopologues. The CL molecular mass was obtained from the quadrupole mass spectrometer (QqQ) mass spectrometer. The doubly charged CL plus-one isotopologues were used to quantify individual CL molecular species as previously described (50, 73). The results are expressed as nmol/mg protein and represent the mean ± standard deviation from three independent isolations of mitochondria. The ion peaks of CL molecular species that constituted less than 0.01 nmol/mg protein as determined by a QqQ mass spectrometer have been omitted from the Table.