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Loss of HRASLS3 does not affect mitotic clonal expansion of 3T3-L1 preadipocytes. Undifferentiated 3T3-L1 stable transformants were plated at a density of 50,000 cells per well. Dexamethasone, methylisobutyl xanthine, and insulin (DMI) was added at time of confluence. Cells were trypsinized and analyzed at the indicated time after the addition of DMI. DNA content was determined by propidium iodide staining using FACS.
