Fig. 8.
Effect of filipin on ABCA1-mediated newly synthesized cholesterol efflux. ABCA1 expression was first enhanced by incubating cells for 18 h in the presence or absence of 2 μM T0901317. Following this incubation, the basal medium was replaced with medium containing 3 μg/ml apoA-I. The apical medium was replaced with medium containing 10 μCi [3H]mevalonate with or without 2 μg/ml filipin. T0901317 was added back to cells that had it originally. At the end of 18 h, the amount of labeled cholesterol found within cells (upper bars) and that recovered in the basal medium (lower bars) were estimated. The data represent the mean ± SE of 4 dishes. *P < 0.05 versus cells incubated without filipin but with T0901317.