Figure 1.

Schematic representation of in vitro and in vivo reconstitution methods to produce a 4 subunit recombinant protein complex. (a) With in vitro reconstitution, each of the 4 subunits is expressed and purified individually before combining to form the complex, which must be further purified from partial or misfolded complexes. (b) In contrast, in vivo reconstitution requires only a single expression step and one round of purification steps to produce purified the 4 subunit complex.