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Polycistronic expression vector worksheet
| component | 1 | 2 | 3 | 4 | ||
|---|---|---|---|---|---|---|
| gene name | ||||||
| pST50Trc1–4 | nonfusion 5’ | NdeI | ||||
| fusion 5’ | BamHI | |||||
| fusion 3’ | NgoMIV | |||||
| nonfusion 3’ | BsrGI | |||||
| pST44 cassette 1 | 5’ site | XbaI | ||||
| alt 5’ site | NheI | |||||
| blunt site | StuI | |||||
| 3’ site | BglII | |||||
| pST44 cassette 2 | 5’ site | EcoRI | ||||
| alt 5’ site | MfeI | |||||
| blunt site | SmaI | |||||
| 3’ site | HindIII | |||||
| pST44 cassette 3 | 5’ site | SacI | ||||
| blunt site | EcoRV | |||||
| 3’ site | KpnI | |||||
| pST44 cassette 4 | 5’ site | BspEI | ||||
| blunt site | NruI | |||||
| 3’ site | MluI | |||||
| alt 3’ site | BssHII | |||||
