T100 buffer
20 mM Tris-Cl pH 8.0	1 ml 1 M Tris-Cl pH 8.0
100 mM NaCl	1 ml 5 M NaCl
0.5 mM EDTA, Na2	50 μl 0.5 M EDTA, Na2
1 mM benzamidine	50 μl 1 M benzamidine
10 mM 2-mercaptoethanol	35 μl stock (14.4 M) 2-mercaptoethanol
	MilliQ water to 50 ml